Anti-p53 (L330) Antibody

This rabbit polyclonal antibody targets the p53 tumor suppressor protein (TP53), one of the most frequently mutated genes in human cancer and a central regulator of the DNA damage response, cell cycle arrest, and apoptosis. Raised against an epitope designated L330, it is supplied by Antibodies.com as a 100 µl vial of unconjugated IgG at 1 mg/ml in phosphate buffered saline (approx. pH 7.2) with 0.05% sodium azide as preservative, a formulation suited to short-term use at 4 °C with minimal freeze-thaw cycling. The reagent has been validated for western blot, immunohistochemistry, and immunofluorescence, and it cross-reacts with p53 from human, mouse, and rat samples, supporting comparative studies of tumor biology and stress signaling across species. Because it is supplied unconjugated, researchers can pair it with HRP-, biotin-, or fluorophore-labeled secondary antibodies matched to their preferred detection chemistry. Typical applications include verifying TP53 status in tumor tissue sections, monitoring p53 stabilization in response to genotoxic stress in cultured cells, and confirming expression or post-translational modification by immunoblotting whole-cell lysates.