Anti-p53 (K386) Antibody

This rabbit polyclonal reagent from Antibodies.com targets p53, a tumor suppressor protein widely studied for its central role in the DNA damage response, cell-cycle arrest, and apoptosis. The antibody recognizes the lysine 386 (K386) residue, an acetylation site whose modification state influences p53 transcriptional activity, DNA-binding affinity, and protein stability under genotoxic and oxidative stress.

Supplied unconjugated at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, the product is packaged as a 100 µl vial. Validated applications include western blot, immunohistochemistry, and immunofluorescence, with reactivity confirmed against human, mouse, and rat samples.

Researchers use this antibody to probe post-translational regulation of p53 in whole-cell lysates, fixed tissue sections, and cultured cells. For best results, aliquot upon arrival and store at -20°C; avoid repeated freeze-thaw cycles to preserve binding activity. Short-term working aliquots may be kept refrigerated for routine use.