Anti-p53 (C-terminus) Antibody

The p53 tumor suppressor is encoded by TP53, the most frequently mutated locus in human cancer. Reliable detection of the full-length protein underpins oncology research, DNA damage response studies, and apoptosis pathway investigations. This Mouse monoclonal antibody from Antibodies.com recognizes an epitope in the C-terminal regulatory domain of human p53, a region encompassing the tetramerization domain and a cluster of post-translational modification sites. C-terminal targeting is widely used to discriminate full-length wild-type p53 from the many N-terminally truncated or conformationally altered variants seen in tumor samples.

Each vial contains 100 µl of purified monoclonal antibody in a buffer of 0.1M Tris-Glycine at pH 7.4 with 150 mM NaCl, 0.2% sodium azide as a preservative, and 50% glycerol for cold storage. The unconjugated format leaves detection chemistry open to user choice, accommodating different secondary antibodies and chromogenic, fluorescent, or chemiluminescent readouts in Western blot workflows. Reactivity has been confirmed against both Human and Mouse p53, enabling cross-species comparisons between patient-derived material and genetically engineered mouse models of tumorigenesis.

Mouse host and monoclonal clonality ensure reproducible immunoblotting lot after lot, while C-terminal epitope coverage pairs naturally with N-terminal p53 antibodies used to map truncation breakpoints. Store at -20 degrees C in the supplied glycerol buffer, aliquot on first thaw to limit freeze-thaw cycles, and protect from light to preserve binding activity.