Anti-p53 Antibody

Available through the Antibodies.com catalogue, this rabbit polyclonal reagent is raised against p53 (also known as TP53), the well-characterised 53 kDa tumour-suppressor protein, and is supplied as an unconjugated liquid suitable for sensitive detection of endogenous human p53. The antibody is produced in rabbit as a polyclonal IgG preparation, ensuring broad epitope coverage, and is provided in a 50 µl format that can be used directly in standard immunodetection workflows. Validated applications include Western Blot (recommended dilution range 1:500–1:1000) and ELISA (recommended dilution 1:10000), making it a reliable primary detection reagent for studies of p53 expression, stability and post-translational regulation in human samples. The immunoglobulin fraction is purified from rabbit serum by antigen affinity chromatography using the immunizing p53 peptide, which minimises non-specific background and provides lot-to-lot consistency. As indicated on the Antibodies.com product specification, the reagent is unconjugated, allowing researchers to pair it with their preferred secondary antibody or detection system (HRP, AP, biotin, fluorophore) for flexible assay design. Recommended storage is at –20 °C in aliquots to preserve activity across multiple freeze–thaw cycles, and the product is intended for research use only.