Anti-p53 Antibody

The p53 tumour suppressor is a 53 kDa nuclear phosphoprotein and master regulator of the DNA damage response, governing cell cycle arrest, apoptosis, senescence, and DNA repair through its activity as a sequence-specific transcription factor. This rabbit-derived polyclonal reagent from Antibodies.com targets conserved epitopes on human p53, supporting a broad range of investigational contexts in cancer biology, stress signalling, and apoptosis research.

Validated for Western blot and ELISA workflows, the antibody is supplied as an unconjugated antiserum at 100 µl per vial, allowing flexible incorporation into standard immunoblotting or plate-based detection schemes. Its reactivity profile has been confirmed against human and non-human primate (monkey) samples, supporting translational studies that compare tumour-derived and normal tissue specimens. Because p53 is among the most frequently mutated genes in human malignancy, this reagent is useful for interrogating wild-type versus mutant expression patterns, assessing downstream transcriptional targets, and characterising cellular responses to genotoxic agents.

Researchers commonly deploy the antibody to probe total p53 levels by Western blot following induction with DNA-damaging compounds such as etoposide, doxorubicin, or ultraviolet radiation, and to quantify p53 antigen in cell lysates by ELISA. Rabbit polyclonal reagents also offer the advantage of recognising multiple epitopes on the target, improving tolerance to partial degradation or post-translational modification during sample preparation. For long-term performance, aliquot the reagent, store at -20 degrees C, avoid repeated freeze-thaw cycles, and protect from light to preserve antibody activity.