Anti-p53 Antibody

Raised in rabbit, this unconjugated polyclonal antibody recognizes p53, a tumor suppressor transcription factor that governs cell cycle arrest, apoptosis, DNA repair, and cellular senescence. Mutations in the TP53 gene rank among the most frequent genetic alterations identified in human cancers, positioning p53 at the center of oncology research, DNA damage response studies, and investigations of genomic stability.

Supplied as 100 µl of polyclonal antiserum, the reagent has been validated for Western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. It detects p53 in human, rat, and monkey samples, enabling comparative studies across cell lines and tissue models. The unconjugated format leaves labeling open, so the antibody can be paired with fluorophore-conjugated, biotinylated, or HRP-coupled secondary reagents depending on the detection platform.

Distributed by Antibodies.com, the antibody is applied in studies of post-translational modification of p53, including phosphorylation, acetylation, and ubiquitylation events that tune its stability and transcriptional activity. Researchers commonly deploy it on paraffin-embedded tissue sections, frozen sections, and cultured cells to assess subcellular localization, mutational status, and stress-induced accumulation in normal versus transformed samples.