Anti-p53 Antibody

Raised in rabbit against the tumor suppressor p53, this unconjugated polyclonal primary antibody from Antibodies.com is supplied as 100 µl of ready-to-use reagent and validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. The antibody recognizes the p53 protein, also called TP53 or cellular tumor antigen p53, a 53 kDa transcription factor encoded by the TP53 gene that governs cell cycle arrest, apoptosis, senescence, and the DNA damage response.

Reactivity is confirmed on human samples and on monkey (Macaque) tissue, supporting comparative oncology studies, tumor biopsy characterization, and investigations of mutant versus wild-type p53 expression in transformed cell lines. Because the polyclonal format presents multiple epitopes across the p53 molecule, the reagent remains useful on samples where missense mutations, truncations, or post-translational modifications might conceal a single linear epitope. Supplied unconjugated, the antibody can be paired with secondary reagents of the researcher's choice for chromogenic or fluorescent readouts on western blot membranes, formalin-fixed paraffin-embedded tissue sections, and cultured cell preparations. Store refrigerated in working aliquots to preserve activity across repeated freeze-thaw cycles.