Anti-p53 Antibody

This Mouse IgG2b monoclonal antibody from Antibodies.com is raised against the p53 tumor suppressor and is supplied as a 50 µL, unconjugated, ready-to-use reagent. Produced in mouse and validated for Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (FC) applications, the reagent targets human p53 and is intended for researchers studying cell-cycle regulation, DNA-damage responses, and oncology biomarkers. The antibody is formulated in 1× TBS (pH 7.4) supplemented with 1% BSA and 40% glycerol, with 0.05% sodium azide included as a preservative, providing a stable glycerol-based buffer suitable for short-term storage at 4 °C and longer-term storage at -20 °C. This preparation makes it convenient for routine immunodetection workflows across multiple platforms, since the same lot can be used in Western blotting, fixed-cell immunocytochemistry, tissue section immunohistochemistry, and intracellular flow cytometry experiments. As an unconjugated primary antibody, it is fully compatible with user-selected secondary detection reagents (HRP, fluorophore, or biotin-based), enabling flexible experimental design when studying TP53 expression, mutation-associated accumulation, or stress-induced stabilization of p53 in human cell lines and tissue specimens. Lot-to-lot consistency and the well-characterized anti-p53 specificity make this Antibodies.com reagent a useful addition to any laboratory working on tumor suppressor biology, apoptosis pathways, or p53-targeted drug screening.