Anti-p53 Antibody

A recombinant rabbit monoclonal antibody raised against p53 (TP53), the tumor suppressor protein most frequently mutated in human cancers. Supplied by Antibodies.com as an unconjugated reagent in 100 µl format, this clone is validated for western blot and immunocytochemistry/immunofluorescence workflows on human samples.

The antibody is provided in a stabilizing buffer composed of TBS (pH 7.4) with 1% BSA and 40% glycerol, with 0.05% sodium azide added as preservative. This formulation supports reproducible staining of endogenous p53 in fixed cell preparations and clean detection of the ~53 kDa band in western blot lysates derived from cultured human cell lines.

Researchers investigating DNA damage response, apoptosis regulation, or oncogenic signaling pathways commonly deploy this reagent to monitor p53 stabilization following genotoxic stress, map subcellular localization patterns, and confirm expression status in tumor versus matched normal controls. Storage at 4°C in the supplied buffer is recommended for short-term use; aliquoting before freezing at -20°C extends working shelf life for ongoing projects.