Anti-p53 Antibody

Raised in rabbit against the p53 tumor suppressor, this polyclonal antibody from Antibodies.com enables detection of endogenous human p53 across Western blot, immunohistochemistry, and immunofluorescence workflows. p53 orchestrates the cellular response to DNA damage by directing cell cycle arrest, apoptosis, senescence, and DNA repair programs, making reliable detection reagents essential to oncology, genotoxic stress, and chemotherapeutic response studies.

Each vial contains 100 µl of antibody supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant. The unconjugated polyclonal format allows users to pair the reagent with their preferred secondary antibody and detection chemistry. Human reactivity is established, supporting work on tumor-derived cell lines, primary tissue sections, and fixed specimens where nuclear accumulation of p53 is routinely scored as a marker of mutation or stress.

Typical applications include Western blot characterization of p53 stabilization following irradiation or drug treatment, immunofluorescence imaging of nuclear foci in cultured cells, and immunohistochemistry of formalin-fixed paraffin-embedded tumor biopsies to assess mutant p53 accumulation. Store aliquots at -20 °C in the supplied glycerol buffer to minimize freeze-thaw cycles and preserve reagent integrity across multiple experiments.