Anti-p53 (acetyl K382) Antibody [2B7E4]

This clone 2B7E4 monoclonal antibody from Antibodies.com specifically recognizes the acetylated lysine 382 (K382) residue of the human p53 tumor suppressor protein. K382 acetylation is a post-translational modification deposited primarily by the p300 and CBP acetyltransferases and serves as a key marker of p53 transcriptional activation following genotoxic stress, including exposure to UV irradiation and chemotherapeutic agents such as etoposide.

The unconjugated IgG1 reagent is supplied as a 100 µg liquid aliquot suitable for western blot detection of acetylated p53 in whole-cell lysates, with recommended working dilutions typically in the 1:500 to 1:2000 range. Researchers commonly use this reagent to monitor DNA damage response signaling, apoptosis induction, and the functional activation status of p53 in tumor-derived cell lines and patient samples.

For optimal preservation of antibody activity, aliquot upon receipt and store frozen at -20 °C, avoiding repeated freeze-thaw cycles.