Anti-p53 (Ab-6) Antibody

Designed for Western blotting (WB), this Antibodies.com primary antibody is a rabbit polyclonal, unconjugated antibody directed against human p53. It detects the endogenous level of total p53 protein.

Produced by immunizing rabbits with synthetic peptide and KLH conjugates, the antibody was purified by affinity chromatography using an epitope-specific peptide. The immunogen is a peptide sequence around aa.4~8 (P-Q-S-D-P) derived from human p53. The reported concentration is 1.0 mg/mL. The formulation is phosphate-buffered saline, pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended Western blotting dilution is 1:500~1:1000, and the predicted molecular weight is 53kd.