Anti-p53 (Ab-15) Antibody

The p53 tumor suppressor protein, encoded by the TP53 gene and frequently mutated across human cancers, is a central focus of oncology and cell biology research. This rabbit polyclonal antibody from Antibodies.com is raised against the Ab-15 epitope region of p53 and is supplied as 100 µl of unconjugated serum suitable for multiple detection platforms.

Researchers commonly apply it to Western blot analysis of cell lysates, immunohistochemistry on formalin-fixed tissue sections, and immunofluorescence imaging of cultured cells to visualize endogenous p53 localization and expression patterns. The antibody reacts with human p53 and is validated for use in studies of DNA damage response, cell cycle regulation, apoptosis induction, and senescence pathways.

Its polyclonal nature allows recognition of multiple epitopes on the target protein, which can be advantageous when monitoring changes in p53 expression or mutation status across different experimental conditions. The unconjugated format provides flexibility for laboratories to pair the antibody with their preferred secondary detection reagents and fluorophore or enzyme conjugates.