Anti-P4HTM Antibody

This rabbit polyclonal antibody targets P4HTM (Prolyl 4-Hydroxylase, Transmembrane), a membrane-anchored enzyme that resides in the endoplasmic reticulum and contributes to cellular oxygen-sensing by hydroxylating proline residues. Produced by Antibodies.com as a research-grade primary antibody, it is raised in rabbit, is of the IgG isotype, and is supplied as an unconjugated preparation in 100 µl, ready for downstream detection with secondary reagents of choice. The reagent is validated for Western blot (WB) and has been characterized for reactivity against human and mouse samples, supporting work in cell biology, hypoxia signaling, and HIF-pathway research where P4HTM activity is under investigation. For western blot, the antibody is typically applied to lysates prepared from cultured mammalian cells or relevant tissue, and detection is performed with standard HRP- or fluorophore-conjugated anti-rabbit secondaries depending on the chosen imaging platform. Routine dilute-and-store handling as single-use aliquots stored at refrigerated temperature helps preserve titer across repeated experiments, and the unconjugated format allows the end user to pair it with their preferred label.