Anti-P4HB Antibody

This primary antibody from Antibodies.com is a rabbit polyclonal antibody targeting P4HB. It is supplied as an unconjugated IgG in liquid form, and the manufacturer reports a target molecular weight of 57 kDa. The brand listing indicates reactivity with human samples, while the manufacturer additionally lists reactivity with mouse and rat samples.

The immunogen is a recombinant fusion protein corresponding to amino acids 329–508 of human P4HB (NP_000909.2), and the antibody is purified by affinity chromatography. The formulation is supplied in phosphate-buffered saline, pH 7.3, containing 50% glycerol and 0.05% Proclin 300. The product is shipped at 4°C and, upon delivery, should be aliquoted and stored at −20°C, with freeze/thaw cycles to be avoided. The concentration is reported as lot-specific.

Validated applications include Western blot (WB) and immunohistochemistry (IHC), with the manufacturer also listing ICC/IF. Recommended dilutions are 1:500–1:1,000 for WB and 1:50–1:200 for IHC and ICC/IF.