Anti-P4HA3 Antibody

This rabbit polyclonal primary antibody from Antibodies.com recognizes P4HA3 (Prolyl 4-Hydroxylase Subunit Alpha 3), one of three alpha subunits of the prolyl 4-hydroxylase enzyme complex responsible for the post-translational hydroxylation of proline residues within -Xaa-Pro-Gly- repeats of procollagen. That modification is required for stable triple-helix formation of fibrillar collagens and is therefore central to studies of extracellular matrix assembly and connective tissue biology.

Supplied as an unconjugated IgG in a 200 µl volume, the reagent is validated for Western blot detection of the human P4HA3 protein in research lysates. The polyclonal format gives multi-epitope recognition, which can help reduce signal loss if a single epitope is masked. Host species is rabbit, clonality is polyclonal, isotype is IgG, and the conjugate is unconjugated for flexible downstream detection. To preserve activity, aliquot upon arrival, store frozen, and protect from light and repeated freeze-thaw cycles.

Typical use cases include profiling P4HA3 expression across human cell lines and tumor samples, comparing alpha-subunit composition of the prolyl 4-hydroxylase complex, and investigating how altered proline hydroxylation contributes to collagen-related disease models. Reactivity is reported for human samples, and the antibody is intended for research use only.