Anti-p44 / 42 MAP Kinase (Ab-202) Antibody

Phosphorylation-specific detection of ERK1 (p44) and ERK2 (p42) at the dual Thr202/Tyr204 activating motif, delivered as an unconjugated rabbit polyclonal antibody for western blot and immunohistochemistry workflows from Antibodies.com. The reagent was raised against the Ab-202 epitope region and preferentially binds the dually phosphorylated, catalytically active form of these MAP kinases produced by the upstream MEK1/2 cascade, which makes it a widely used readout for growth factor, cytokine, and oncogenic Ras/Raf/MEK/ERK signaling in cell biology research.

Validated on Human, Mouse, and Rat samples, the antibody is supplied as 100 µl of liquid stock and is suitable for immunoblotting of whole-cell lysates as well as chromogenic or fluorescent IHC on fixed tissue sections. As a polyclonal reagent it offers broader epitope coverage than a monoclonal clone, and the unconjugated format allows researchers to couple it to HRP, biotin, Alexa Fluor, or other labels according to their assay design.

Typical uses include tracking the time course of MAPK pathway activation following serum stimulation, drug-induced ERK inhibition studies with MEK inhibitors such as U0126 or PD0325901, and tumor tissue profiling where ERK phosphorylation status is investigated as a marker of pathway activity. Storage follows standard polyclonal antibody handling: short-term at 4 °C, long-term aliquoted and stored frozen to limit freeze-thaw loss of titer.