Anti-p38 (phospho-Y182) Antibody

Offered through the Antibodies.com catalogue, this affinity-purified reagent is a rabbit polyclonal antibody raised against p38/MAPK14 when phosphorylated at tyrosine 182 (phospho-Y182). It is supplied as a 50 µl aliquot at approximately 1 mg/ml in phosphate-buffered saline (PBS) containing 0.1% sodium azide and 50% glycerol, with a reported purity of ~95% by SDS-PAGE. The unconjugated antibody specifically detects endogenous levels of MAPK14 only when phosphorylated at Tyr182, and the immunogen is a synthetic phosphopeptide derived from human p38/MAPK14 around the Y182 site. Recommended applications on the supplier datasheet are Western blot (WB) and immunofluorescence (IF), and the target is reported at an apparent molecular weight of ~42 kDa. The host species is rabbit, the clonality is polyclonal, and the reagent is unconjugated, with reactivity validated in Human, Mouse and Rat samples. Short-term storage is compatible with 4 °C, while the glycerol-containing buffer allows longer-term storage at -20 °C with avoidance of repeated freeze/thaw cycles. For research use only; not intended for diagnostic or therapeutic applications. This reagent from Antibodies.com is suited to studies of MAPK14 / p38 signaling, stress kinase activation, and phosphorylation-dependent readouts in cell and tissue lysates.