Anti-p38 (phospho-T180) Antibody

Raised in rabbit, this polyclonal reagent from Antibodies.com recognizes p38 MAPK only when phosphorylated at threonine 180, the activation-loop residue whose modification drives downstream stress and inflammatory signaling. The unconjugated IgG preparation is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, and is validated for Western blot detection of the active kinase across human, mouse, and rat samples. Each vial delivers 100 µl of antibody, sufficient for multiple membrane probing experiments examining stimuli such as UV irradiation, osmotic shock, or cytokine exposure that trigger p38 pathway engagement. Researchers studying MAPK cascades in cancer, neurodegeneration, cardiovascular disease, and immune cell differentiation rely on phospho-specific reagents like this to distinguish basal expression from stimulus-induced activation, since total p38 levels often remain constant while phosphorylation status shifts within minutes of treatment. The polyclonal format provides broader epitope coverage than monoclonal alternatives, helping maintain signal in denatured, reduced lysate conditions typical of SDS-PAGE workflows. Storage at 4 °C is appropriate for short-term use; for longer intervals, aliquoting and freezing at -20 °C preserves activity and protects against repeated freeze-thaw cycles that can degrade antibody performance. Pairing this phospho-T180 reagent with a total p38 loading control on parallel blots allows accurate normalization of pathway activation between treated and untreated conditions.