Anti-p38 Antibody

This polyclonal antibody from Antibodies.com targets p38 MAPK, a serine/threonine kinase encoded by the MAPK14 gene that mediates cellular responses to environmental stress, pro-inflammatory cytokines, and osmotic shock. Raised in rabbit and supplied as 100 µl of unconjugated antiserum, the reagent supports western blot detection of phosphorylated and total p38 from cultured mammalian cells or tissue lysates, immunohistochemistry on formalin-fixed paraffin-embedded sections, and ELISA-based quantification of p38 in experimental samples. The antibody recognizes p38 in human, mouse, and rat samples, making it useful for cross-species comparative studies of inflammation, apoptosis, and cell cycle regulation.

p38 MAPK sits at the center of the stress-activated MAPK cascade, downstream of MKK3 and MKK6, and is itself a substrate for upstream MAP2Ks that activate it through dual threonine and tyrosine phosphorylation on the Thr180-Tyr182 activation motif. Researchers studying signal transduction, cytokine signaling, and innate immunity routinely probe p38 activation in response to TNF-alpha, IL-1, and UV exposure, tracking phosphorylation state as a marker of pathway engagement. The unconjugated format allows users to pair this primary antibody with HRP- or fluorophore-conjugated secondary reagents tailored to their downstream detection platform, including chemiluminescent western blot and chromogenic IHC workflows.