Anti-p38 Antibody

Primary antibody reagent for studying the p38 MAPK stress-activated kinase signaling pathway. This mouse monoclonal reagent from Antibodies.com targets the p38 mitogen-activated protein kinase, a serine/threonine kinase central to inflammatory cytokine signaling, cellular stress responses, and apoptosis regulation.

Validated across three core applications: Western blot for detecting p38 expression in cell and tissue lysates, immunocytochemistry for visualizing kinase localization in cultured cells, and immunohistochemistry for mapping distribution in fixed tissue sections. The antibody shows reactivity with both human and mouse samples, enabling translational workflows that bridge murine disease models and human specimen analysis.

Supplied as 100 µl of purified Mouse IgG2a in a 1×TBS (pH 7.4) buffer containing 1% BSA and 40% glycerol, preserved with 0.05% sodium azide. The glycerol-stabilized formulation supports reliable storage under standard refrigeration conditions and tolerates routine freeze-thaw cycling when aliquoted into working stocks. Unconjugated format provides flexibility for downstream detection chemistries, including HRP-, fluorophore-, or biotin-conjugated secondary reagents tailored to specific assay platforms.

The p38 MAPK pathway is widely investigated in inflammatory disease biology, oncology signal transduction, and cellular stress research. Investigators use this reagent to probe pathway activation, monitor kinase expression under experimental perturbations such as cytokine treatment or inhibitor exposure, and characterize tissue-level distribution patterns in disease and control samples.