Anti-p27Kip1 (Ab-10) Antibody

Offered through Antibodies.com, this affinity-purified polyclonal reagent was generated in rabbit against a synthetic peptide corresponding to residues around aa. 8–12 (N-G-S-P-S) of human p27Kip1, also known as CDKN1B, cyclin-dependent kinase inhibitor 1B, or KIP1. Supplied as a 50 µl liquid at 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, the unconjugated IgG is intended for research use only and is recommended for Western blotting at 1:500–1:1000 and immunohistochemistry at 1:50–1:100. The antibody selectively recognizes the endogenous total p27Kip1 protein (predicted molecular weight ≈ 27 kDa) in human, mouse and rat samples, making it suitable for studies of cell-cycle regulation across these species. For day-to-day handling, vials are shipped at 4 °C and should be aliquoted for storage at –20 °C, with freeze/thaw cycles avoided to preserve activity. Polyclonal antibodies.com preparations such as this one are produced by immunizing rabbits with the target peptide–KLH conjugate and then purified by epitope-specific affinity chromatography, providing a robust reagent for laboratories investigating cyclin-dependent kinase inhibition, G1/S checkpoint control and related pathways.