Anti-p27 Kip1 (phospho-T198) Antibody

The p27 Kip1 cyclin-dependent kinase inhibitor regulates G1/S transition and exit from quiescence, and threonine-198 phosphorylation is the modification that relocates p27 from the nucleus to the cytoplasm, where it has been linked to aggressive behavior in breast, prostate and other solid tumors. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the phospho-T198 epitope and is intended for Western blot detection of the phosphorylated form in human, mouse and rat samples. Each vial is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, giving 100 µl of ready-to-use stock. Dilute in standard blocking buffer for immunoblotting of whole-cell or tissue lysates; the unconjugated format lets researchers pair it with their preferred HRP- or fluorophore-labeled secondary antibody. Investigators working on Akt-dependent signaling, HER2-positive disease, cell cycle re-entry, or cytoplasmic p27-driven metastatic programs use this reagent to separate the tumor-suppressive nuclear pool from the phospho-T198 cytoplasmic pool in tumor samples and cultured cells.