Anti-p27 Kip1 Antibody

This rabbit polyclonal antibody targets p27 Kip1 (CDKN1B), a cyclin-dependent kinase inhibitor that serves as a critical regulator of the G1/S cell cycle transition and a well-characterized tumor suppressor in many tissue types. Manufactured by Antibodies.com as a primary antibody reagent, it is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, in a 50 µl vial ready for use across multiple western blot and immunohistochemistry experiments.

Validated for WB and IHC workflows, the antibody shows reactivity with human, mouse, and rat samples, making it a versatile tool for cell cycle, oncology, and signal transduction studies across multiple model systems. The unconjugated format allows researchers to pair it with custom secondary antibodies or label it directly for specific detection chemistries. For best results, aliquot upon arrival and store frozen at -20°C; avoid repeated freeze-thaw cycles to preserve binding activity over the shelf life of the reagent.

Because the target regulates CDK2/Cyclin E activity and responds to upstream cues such as FoxO transcription factors and SCF-Skp2-mediated proteasomal degradation, this reagent supports investigations into proliferation arrest, differentiation, apoptosis, and tumor biology in both in vitro cell culture models and tissue sections.