Anti-p27 Kip1 Antibody

The p27 Kip1 protein, encoded by the CDKN1B gene, is a cyclin-dependent kinase inhibitor that halts cell cycle progression at the G1/S checkpoint by binding cyclin-CDK2 complexes. This rabbit polyclonal reagent from Antibodies.com is raised against p27 Kip1 and validated for Western Blot (WB) and immunohistochemistry across human, mouse, and rat samples.

Supplied as 100 µl of unconjugated antibody at 1 mg/ml in a vial containing PBS, 0.1% sodium azide, and 50% glycerol, the polyclonal antibody gives broad coverage of multiple epitopes for detection of endogenous p27 Kip1 in lysates and fixed tissue sections. Researchers studying tumor suppression, cell cycle exit, differentiation programs, and apoptosis-related signaling rely on p27 Kip1 as a marker to assess proliferative status in normal versus transformed cells.

The glycerol-containing buffer protects the antibody during repeated freeze-thaw cycles when stored at -20 °C, and the 100 µl volume is convenient for aliquoting into single-use portions. Standard uses include western blot analysis of whole-cell extracts to monitor cyclin-dependent kinase inhibitor levels and immunohistochemistry on formalin-fixed paraffin-embedded tissues for spatial mapping of p27 Kip1 expression within tumor microenvironments.

Characterized reactivity with mouse, rat, and human p27 Kip1 enables comparative oncology and developmental biology studies. The unconjugated format allows conjugation to HRP, biotin, fluorophores, or other detection chemistries, supporting flexibility across immunofluorescence, immunoprecipitation, and chromogenic detection protocols.