Anti-p27 Antibody

Manufactured by Antibodies.com, this affinity-purified rabbit polyclonal reagent is raised against the cyclin-dependent kinase inhibitor p27 (also known as KIP1) and is validated for Western blot (WB) and ELISA workflows on human samples. The immunogen is a synthesized peptide derived from human p27 around the non-phosphorylation site of T198, allowing the antibody to detect endogenous levels of p27 protein in cell lysates and recombinant preparations. Supplied as 50 µL of an unconjugated liquid formulation at 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, the antibody is polyclonal in clonality, isotype IgG, and is recommended for short-term storage at 4 °C with longer term aliquoting and freezing at -20 °C to preserve activity. It is intended for research use only and is not approved for diagnostic or therapeutic applications, with shipping dispatched from St. Louis, MO and a typical lead time of 6–9 business days. The reagent pairs well with standard HRP- or fluorophore-conjugated secondary antibodies and is suitable for studies of cell-cycle regulation, proliferation, and CDK inhibition in human cell lines and tissue samples.