Anti-p27 Antibody

Target the cyclin-dependent kinase inhibitor p27 (also called CDKN1B and p27Kip1) with this rabbit polyclonal antibody raised against the human p27 protein. p27 acts as a key negative regulator of the cell cycle at the G1/S transition by binding and inhibiting cyclin D-CDK4 and cyclin E-CDK2 complexes, and loss of p27 is widely studied as a prognostic marker in cancer biology, particularly in breast, prostate, and colorectal tumor research. Antibodies.com supplies this reagent as a 100 µl vial of unconjugated polyclonal antiserum suited for Western blot and ELISA workflows. The antibody is validated for reactivity with human p27, making it appropriate for investigations into cell cycle arrest, apoptosis signaling, and tumor suppressor pathways in research use only contexts. Researchers commonly deploy it to detect endogenous p27 levels in cell lysates following growth factor withdrawal, pharmacological CDK inhibition, or siRNA knockdown experiments, and to probe p27 induction by agents such as rapamycin, lovastatin, and TGF-β. Each lot is supplied as a liquid aliquot ready for dilution into blocking buffer, and the polyclonal format enables recognition of multiple epitopes on the target for robust signal across a range of lysate loads in reducing SDS-PAGE gels.