Anti-p21 (FL) Antibody

Manufactured by Antibodies.com as a primary antibody for cell-cycle research, this rabbit polyclonal reagent targets full-length p21 (CDKN1A/CIP1/WAF1) and is supplied as a 50 µl aliquot of an unconjugated IgG formulated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol. It was raised against recombinant full-length human p21 and affinity-purified to >95% purity by SDS-PAGE, yielding an antibody of ~21 kDa on blot that detects endogenous p21 in Human, Mouse and Rat samples. Validated applications include Western Blot (1:2000–1:5000), Immunohistochemistry (1:50–1:200) and Immunofluorescence/Immunocytochemistry, making it suitable for tracking p21 induction by stress stimuli and p53-dependent G1/G2 checkpoint signalling. The unconjugated format allows flexible labelling, and the reagent is stable for short-term storage at 4 °C with long-term aliquots held at −20 °C, avoiding repeated freeze-thaw cycles.