Anti-p21 Antibody

Manufactured by Antibodies.com, this affinity-purified reagent is a rabbit polyclonal antibody raised against a synthetic peptide derived from human p21 surrounding the non-phosphorylation site of threonine 145, enabling selective detection of endogenous p21 protein across multiple experimental platforms. The unconjugated antibody is supplied as a liquid formulation in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide at a concentration of 1 mg/mL, and is offered in 50 µL and 100 µL volumes. It has been validated for ELISA, Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC), and it cross-reacts with samples from human, mouse, and rat. The polyclonal host species (rabbit) and unconjugated format allow researchers to pair the reagent with custom fluorophore or enzyme labelling schemes, while affinity-chromatography purification using the epitope-specific immunogen helps minimise off-target binding. For routine handling, aliquots should be stored at –20 °C and protected from repeated freeze-thaw cycles to preserve activity. As a primary antibody directed against the cyclin-dependent kinase inhibitor p21 (CDKN1A), the reagent is well suited to studies of cell-cycle arrest, DNA-damage response, cellular senescence, and tumour-suppressor pathways, fitting comfortably into standard immunodetection workflows in molecular and cell biology laboratories.