Anti-P-Selectin Antibody

P-Selectin (CD62P) is a type I transmembrane glycoprotein stored in the alpha granules of platelets and Weibel-Palade bodies of endothelial cells. This polyclonal antibody from Antibodies.com is raised in rabbit against human P-Selectin and is supplied as an unconjugated reagent in a 100 µl volume. The antibody recognizes an epitope conserved across human, mouse, and rat sequences, making it useful in comparative studies of rodent inflammation models and human clinical specimens.

Recommended applications include Western blot for detection of full-length and soluble P-Selectin in cell lysates, and ELISA for quantification of circulating levels. The polyclonal format provides recognition of multiple epitopes, which is helpful when target conformation may vary between activated and resting cells. Common research contexts involve leukocyte rolling, thrombosis, atherosclerosis, sepsis, and inflammatory cytokine signaling, where rapid mobilization of P-Selectin to the cell surface drives neutrophil and monocyte recruitment.

For handling, aliquot the reagent and store refrigerated; avoid repeated freeze-thaw cycles to preserve antibody activity. The unconjugated format allows end-users to couple the antibody to HRP, biotin, fluorophores, or gold conjugates depending on the chosen detection platform.