Anti-ODF2 Antibody

ODF2 (Outer Dense Fiber 2) is a cytoskeletal scaffolding protein best known as a structural component of the outer dense fibers that run along the axoneme of the mammalian sperm flagellum. Beyond its role in flagellar assembly, ODF2 also localizes to centrosomes and basal bodies, where the cenexin splice variant contributes to microtubule anchoring and primary cilium formation. This Antibodies.com reagent is a rabbit polyclonal antibody raised against ODF2, supplied as an unconjugated 100 µL liquid aliquot, and validated for Western blot and immunohistochemistry on human samples. Researchers studying male reproductive biology, spermatogenesis defects, motile cilia function, or centrosome and centriole organization use this reagent to detect the full-length ~95 kDa protein in testis lysates, fixed tissue sections, and cultured cells. The polyclonal format increases the likelihood of recognizing multiple epitopes across isoforms, which is useful when probing truncated variants or post-translationally modified forms. For Western blot, typical sample preparation involves homogenizing tissue or pelleting cells in RIPA buffer followed by SDS-PAGE separation; for IHC, antigen retrieval on formalin-fixed paraffin-embedded sections improves signal in densely packed spermatid and Sertoli cell contexts. Store refrigerated at 4 °C for short-term use, or aliquot and freeze at -20 °C for longer-term stability, avoiding repeated freeze-thaw cycles. The antibody is intended for research use only.