Anti-Oct-3 / 4 Antibody

Raised in rabbit and affinity-purified by epitope-specific immunogen chromatography, this polyclonal reagent from Antibodies.com is designed to detect endogenous levels of Oct-3 / 4 (also known as POU5F1, Oct4, OCT3, and OTF3), a key transcription factor associated with pluripotency in embryonic and induced pluripotent stem cells. The immunogen is a synthesized peptide derived from the internal region of human Oct-3 / 4, ensuring recognition of physiologically expressed protein rather than tagged or overexpressed constructs. Supplied as a 50 µL liquid formulation in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide, the antibody is presented at a working concentration of 1 mg/mL in an unconjugated format, making it well suited for downstream labeling with the researcher's preferred reporter. The immunoglobulin class is IgG, and the preparation has been validated for use in ELISA, Western blot (WB), and immunohistochemistry (IHC) applications across human, mouse, and rat samples. For handling, the vial is shipped at 4 °C and should be aliquoted upon arrival for long-term storage at –20 °C, with repeated freeze/thaw cycles avoided to preserve reagent integrity. Catalog listings for this Antibodies.com product (A35789) typically recommend pairing the primary antibody with HRP, FITC, biotin, or alkaline-phosphatase conjugated goat anti-rabbit IgG secondary reagents, which are listed as compatible companions. The reagent is intended strictly for research use and is not validated for diagnostic or therapeutic applications. Through its regional distributor AFMS in Riyadh, Saudi Arabia, the antibody is offered to life-science laboratories across the Kingdom as part of the Antibodies.com primary antibody portfolio, which emphasizes cost-effective access to high-quality, characterized research tools.