Anti-Oct-3 / 4 Antibody

Oct-3/4 (POU5F1) is a POU-domain transcription factor that governs pluripotency in embryonic stem cells and germ cell lineages, making it one of the most widely used markers for identifying undifferentiated cells in regenerative biology and developmental research. This unconjugated rabbit polyclonal antibody is raised against Oct-3/4 and validated across multiple applications including western blot, immunohistochemistry, and ELISA workflows. Cross-reactivity has been confirmed in human, mouse, and rat samples, supporting translational studies and comparative work between model systems.

The reagent is supplied by Antibodies.com as a 100 µl liquid aliquot of polyclonal antiserum, suitable for both immunostaining of fixed tissue sections and detection of endogenous Oct-3/4 protein levels in cell lysates. Because Oct-3/4 expression is tightly downregulated upon differentiation, the antibody is particularly useful for monitoring stem cell identity, reprogramming efficiency, and germ cell development in vitro. For long-term storage, refrigerate the vial and avoid repeated freeze-thaw cycles to preserve antibody activity across multiple experiments.