Anti-Oct-1 Antibody

The Oct-1 transcription factor, also known as POU2F1 or OTF-1, is a member of the POU-domain family of DNA-binding proteins. It recognizes the canonical octamer motif ATTTGCAT within promoter and enhancer regions and contributes to the regulation of housekeeping genes, immune cell signaling, stress response pathways, and histone H2B expression during early embryonic development. Oct-1 is widely expressed across many tissue types and plays a recognized role in B-cell lineage gene regulation while also modulating the activity of RNA polymerase II and RNA polymerase III complexes.

This rabbit polyclonal antibody from Antibodies.com is raised against the Oct-1 target and is supplied as an unconjugated reagent in a 100 µl volume. It has been validated for Western blot (WB) and immunofluorescence (IF) workflows and is reactive with both Human and Mouse samples, supporting cross-species studies of transcriptional control, lymphocyte biology, and stress-induced gene expression. The polyclonal format allows recognition of multiple epitopes on the transcription factor, which is useful when probing conformational variants or post-translationally modified forms of the protein.

Recommended uses include IF characterization of Oct-1 distribution in cultured cells or tissue sections, examination of nuclear versus cytoplasmic localization patterns, and WB analysis of transcription factor abundance in whole-cell or nuclear extracts. The unconjugated format offers flexibility to pair the antibody with a secondary reporter of the researcher's choice, fitting standard immunodetection protocols in gene regulation, immune signaling, and developmental transcription research.