Anti-OCLN Antibody

Occludin is a ~65 kDa integral membrane tight junction protein central to epithelial and endothelial barrier integrity, and the Anti-OCLN polyclonal antibody from Antibodies.com gives researchers a rabbit-derived IgG reagent for probing this target by Western blot. Generated as an unconjugated antiserum against the OCLN protein, it is validated for detecting endogenous Occludin in lysates from human and mouse samples, supporting comparative work across species and studies that rely on rodent models of barrier disruption.

Supplied as 100 µl of ready-to-use reagent in a single vial, the polyclonal format presents a diverse epitope repertoire, increasing the likelihood of a clean signal even when the antigen is partially degraded or processed. The unconjugated IgG isotype pairs with the laboratory's secondary antibody of choice, supporting chemiluminescent, fluorescent, or HRP-based Western blot detection depending on the imaging system available.

Typical uses include tracking tight junction changes in intestinal, renal, and blood-brain barrier research, monitoring Occludin redistribution after cytokine or pathogen challenge, and following epithelial-mesenchymal transition in cell culture models. Aliquot the reagent upon arrival to limit freeze-thaw cycles, store refrigerated between uses, and protect from light to maintain consistent antibody titre across experiments.