Anti-NUP153 Antibody [R4C8]

The R4C8 clone recognizes an epitope within nucleoporin 153 (NUP153), a ~153 kDa scaffolding component of the nuclear pore complex that mediates mRNA export and karyopherin-cargo translocation across the nuclear envelope. This rat IgG2a reagent is supplied as a liquid formulation in a 200 µg vial and is validated for Western blot, immunocytochemistry (ICC), and ELISA across human, mouse, rat, and monkey samples, producing characteristic nuclear rim and pore-associated staining patterns in fixed cells.

For best results, aliquot upon first use and store at -20°C; avoid repeated freeze-thaw cycles to preserve binding activity. Researchers commonly apply this antibody to studies of nucleocytoplasmic transport, NPC reassembly during mitosis, and viral mechanisms that exploit NUP153 FG-repeat domains during nuclear import. Provided unconjugated, it is compatible with secondary antibody labeling for fluorescence or chromogenic detection in custom assay workflows. Antibodies.com lists each lot with rigorous specificity validation.