Anti-NUMA1 Antibody

This rabbit polyclonal reagent from Antibodies.com targets NUMA1, a nuclear mitotic apparatus protein essential for spindle pole organization and proper chromosome segregation during mitosis. NUMA1 participates in tethering microtubule minus ends to the spindle poles and contributes to mitotic spindle positioning through interactions with the cell cortex.

The unconjugated antibody is supplied as 100 µl of ready-to-use reagent suitable for Western blot and immunohistochemistry workflows using human samples. Researchers studying cell division, aneuploidy, or mitotic checkpoint regulation use NUMA1 immunolabeling to visualize spindle architecture in fixed cells and to confirm expression of the protein in tumor biopsy sections.

The polyclonal nature of the reagent allows recognition of multiple epitopes, increasing robustness when probing denatured lysates in Western blot applications. Each vial should be stored refrigerated and protected from light to preserve antibody activity across repeated freeze-thaw cycles.

Typical applications include immunoblotting of mitotic cell extracts and immunostaining of paraffin-embedded tissue microarrays for studies of mitotic index in cancer research.