Anti-NuMA Antibody

Produced in rabbit and supplied as 100 µl of unconjugated antiserum, this polyclonal reagent from Antibodies.com recognizes the Nuclear Mitotic Apparatus protein (NuMA), a ~240 kDa nuclear and spindle-associated component essential for proper chromosome segregation during cell division. The antibody supports detection of endogenous human NuMA across multiple experimental readouts, including Western blot, immunofluorescence, and immunohistochemistry.

NuMA redistributes dynamically through the cell cycle: it resides in the nucleus during interphase and relocates to spindle poles during mitosis, making the protein a widely used cytological marker for mitotic figures, spindle pole integrity, and post-mitotic nuclear reformation. Researchers studying cell division, spindle assembly checkpoints, and chromosomal instability rely on NuMA immunostaining to score mitotic indices in tissue sections and to evaluate perturbations in microtubule-kinetochore attachment.

Typical applications include Western blot detection of full-length NuMA from cell lysates, immunofluorescence labeling of spindle poles and reassembling nuclei in fixed cells, and immunohistochemical mapping of NuMA distribution in tumor biopsies and developing tissues. The reagent has been used in investigations of aneuploidy, DNA damage response, and structural maintenance of chromatin complexes.

Recommended starting dilutions vary by application: for immunofluorescence on HeLa or U2OS cells, typical working concentrations range from 1:100 to 1:500, while Western blots are commonly probed at 1:500 to 1:1000. Aliquot upon arrival and store at -20°C in working volumes to limit freeze-thaw cycles and preserve antibody activity across multiple experiments.