Anti-NUDT1 Antibody

This polyclonal antibody targets NUDT1 (also called MTH1), a Nudix-family hydrolase that sanitizes the cellular nucleotide pool by cleaving oxidized purine triphosphates such as 8-oxo-dGTP, preventing G>T transversions during DNA replication. Antibodies.com supplies the reagent as a rabbit polyclonal raised against the human sequence, and the antibody cross-reacts with mouse and rat orthologs, enabling parallel studies in rodent tumor models and patient-derived tissue samples.

Laboratories focused on oxidative stress, the DNA damage response, and cancer cell survival rely on this reagent to monitor NUDT1 expression by western blot and to map its subcellular distribution in fixed tissue by immunohistochemistry (IHC). NUDT1 overexpression has been reported across lung adenocarcinoma, breast, and colorectal cancers, where elevated levels help tumor cells cope with reactive oxygen species; the antibody is accordingly used as a benchmark when evaluating MTH1 inhibitors such as TH588 or karonudib in cell-based assays, and as a control for assessing on-target engagement.

Provided as an unconjugated, ready-to-use liquid at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, each vial contains 100 ul. Aliquot on first use to limit freeze-thaw cycles and store refrigerated at 4 degrees C for working stocks; the glycerol formulation remains stable long-term at -20 degrees C. The included sodium azide acts as a preservative, so the reagent is not suitable for workflows that demand azide-free conditions, such as certain live-cell labeling or conjugation chemistries.