Anti-Nucleophosmin Antibody

Raised in rabbit and supplied by Antibodies.com as 100 µl of unconjugated polyclonal antiserum, this Anti-Nucleophosmin Antibody targets the nucleolar phosphoprotein NPM1 (also called B23 or numatrin) and binds the endogenous ~37 kDa target in human, mouse, rat, and monkey samples. Validated workflows include western blot, immunohistochemistry, and ELISA, making the reagent useful for studies of ribosomal biogenesis, centrosome duplication, and the genomic stability roles NPM1 plays in proliferating cells.

Beyond basic cell biology, NPM1 carries diagnostic weight in acute myeloid leukemia research, where mutated forms that mislocalize to the cytoplasm are tracked as a marker in clinical and translational studies. The unconjugated format lets researchers pair the antibody with HRP, biotin, or fluorophore conjugates of their choice for immunofluorescence, chromogenic IHC, or capture-style detection on cell lysates and tissue sections.

For reliable binding activity across multiple experiments, store the vial frozen at -20 °C, prepare working aliquots to limit freeze-thaw cycles, and minimize exposure to room temperature. Protect from light and handle on ice when preparing dilutions for blotting or staining workflows.