Anti-NUCB2 Antibody

From Antibodies.com, this rabbit polyclonal reagent is raised against Nucleobindin 2 (NUCB2), a calcium-binding EF-hand protein best known as the precursor of the anorexigenic neuropeptide Nesfatin-1. NUCB2 is broadly distributed across hypothalamic nuclei involved in appetite control, pancreatic islets, gastric mucosa, and white adipose tissue, making it a frequent target for research into obesity, type 2 diabetes, and neuroendocrine stress signaling.

Validated for Western blot and immunohistochemistry on human samples, the antibody is supplied unconjugated at 100 µL per vial, allowing straightforward adaptation to chemiluminescent, fluorescent, or chromogenic detection workflows. Researchers commonly use it to resolve full-length NUCB2 versus processed Nesfatin-1 fragments, supporting studies on prohormone processing, peptide release, and downstream melanocortin-independent satiety pathways. The polyclonal format offers broader epitope coverage than monoclonal alternatives, which is useful when characterizing cleavage products or probing tissues with variable post-translational modification.

For best results, aliquot the antibody upon arrival to limit freeze-thaw cycles, and store according to the instructions printed on the vial. Each preparation is affinity-purified and quality-controlled by Antibodies.com for the listed applications, helping ensure reproducible band patterns in Western blot and consistent staining across immunohistochemistry experiments.