Anti-NTRK2 (phospho Y515) Antibody

Phosphorylation of the TrkB receptor at tyrosine 515 is the earliest autophosphorylation event triggered by BDNF and NT-4/5 binding, and the Anti-NTRK2 (phospho Y515) reagent from Antibodies.com is a rabbit polyclonal IgG raised specifically against this activated form of the receptor. Supplied as 50 µl of unconjugated antibody, it is validated for immunofluorescence on human, mouse, and rat samples, supporting research into neurotrophic signaling, neuronal differentiation, synaptic plasticity, and downstream survival pathways in the developing and adult nervous system.

Tyrosine 515 phosphorylation is the docking site for the PLCγ1 and Shc adaptor proteins, which in turn recruit the MAPK, PI3K/AKT, and calcium second messenger cascades. By selectively recognizing the phosphorylated epitope, this phospho-specific reagent allows researchers to distinguish activated receptor pools from total TrkB during BDNF stimulation, neuronal activity, or pharmacological intervention, and serves as a useful tool for immunofluorescence co-localization with synaptic markers, postsynaptic density proteins, or other receptor tyrosine kinases in both cell culture and brain tissue preparations.

The polyclonal format increases the likelihood of robust signal across different experimental contexts, while the unconjugated IgG formulation allows users to pair it with their preferred fluorophore-conjugated secondary reagent for multicolor panels. For best results, aliquot on receipt and store frozen in working volumes; protect from light if the antibody is combined with a light-sensitive fluorophore for downstream detection. The reagent is supplied for research use only and is intended for laboratory investigation of neurotrophin biology in academic and industrial settings.