Anti-NTRK2 (phospho Y515) Antibody

A phospho-specific rabbit polyclonal IgG reagent that recognises the Y515 autophosphorylation site of NTRK2 (TrkB), the initiating docking residue created when BDNF engages the receptor and recruits SHC/GRB2 adaptors to launch the Ras-MAPK and PI3K-Akt cascades underlying neuronal survival and synaptic plasticity. The 200 µl vial of unconjugated antiserum is supplied by Antibodies.com as an affinity-purified reagent raised against a synthetic phosphopeptide spanning the human TrkB sequence around tyrosine 515 (P-Q-Y(p)-F-G), detecting endogenous receptor only when the site is phosphorylated. Validated specifically for immunofluorescence on fixed cells or tissue sections, the reagent performs in situ readouts of TrkB engagement at typical working dilutions of 1:100 to 1:500, supporting multiplex co-localisation studies against synaptic compartment markers or downstream phospho-ERK signals. Cross-reactivity with mouse and rat TrkB extends use to standard rodent disease models of mood disorder, neurodegeneration, and TrkB-driven tumour biology alongside human sample banks. The unconjugated format lets researchers pair the primary with a chosen fluorophore-conjugated secondary antibody for custom detection channels. Storage is at -20 °C in working aliquots recommended to limit freeze-thaw cycles and protect from light during incubation. Predicted molecular weight is ~92 kDa for the truncated T1/T2 isoforms and ~145 kDa for the full-length gp145-TrkB receptor, which immunoblot and imaging workflows are expected to resolve accordingly. This reagent is for research use only and is not intended for diagnostic or therapeutic applications.