Anti-NTF3 Antibody

This primary antibody from Antibodies.com is a rabbit polyclonal raised against NTF3 (Neurotrophin 3). It is unconjugated, of IgG isotype, and validated for Western Blot (WB) and Immunohistochemistry (IHC) applications, reacting with Human and Mouse samples [C0, S1].

Manufacturer data indicates the immunogen is a recombinant fusion protein corresponding to amino acids 19–257 of human NTF3 (NP_002518.1) [S1, S2]. The product is supplied as a liquid in Phosphate Buffered Saline, pH 7.3, with 50% glycerol and 0.02% sodium azide [S1, S2]. It is purified by affinity chromatography and shipped at 4°C; aliquots should be stored at -20°C with freeze/thaw cycles avoided [S1, S2]. Recommended dilutions differ between sources: WB at 1:50–1:200 and IHC at 1:50–1:200 in one record [S1], versus WB at 1:500–1:2,000 and IHC at 1:50–1:200 in another [S2].

Sources disagree on the reported molecular weight: one manufacturer record states 29 kDa [S1] while another states 36 kDa [S2]. A third source describes a similar rabbit polyclonal anti-NTF3 antibody supplied at 1.0 mg/mL in PBS pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, detecting endogenous levels of total NTF3 [S3].