Anti-NT5E Antibody

Offered by Antibodies.com, this rabbit polyclonal reagent is raised against NT5E (ecto-5'-nucleotidase, also known as CD73) and is validated for Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA workflows. The antibody is supplied at 50 µl per vial as an unconjugated, affinity-purified IgG preparation at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. The immunogen is a recombinant human NT5E protein, and the reagent specifically detects the endogenous total NT5E protein in samples from human, mouse, and rat origins. Researchers receive a polyclonal host rabbit antibody that has been purified by affinity chromatography using the immunogen, providing high specificity for NT5E in both denatured and native assay formats. For handling, the product is shipped at 4 °C; upon arrival it should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Suitable applications include validation of CD73 expression in tumor immunology studies, immunoblotting of cell lysates, and immunostaining of tissue sections or cultured cells, with reactivity confirmed across human, mouse, and rat species to support translational research programs.