Anti-NSUN6 Antibody

Raised against the RNA methyltransferase NSUN6 in rabbit hosts, this polyclonal IgG reagent from Antibodies.com is supplied as an unconjugated 50 µl vial intended for western blot and immunoprecipitation applications in human and mouse samples.

NSUN6 belongs to the NOL1/NOP2/SUN domain family and installs 5-methylcytosine modifications at position C72 of specific cytoplasmic tRNAs, contributing to post-transcriptional RNA modification patterns that influence translation fidelity and codon-anticodon interactions. The antibody detects endogenous protein expression across mammalian systems, supporting studies of m5C epitranscriptomics in cell line lysates, primary tissue extracts, and RNA-binding protein interactomes. For western blot, common working dilutions fall in the 1:500 to 1:2000 range; immunoprecipitation reactions generally use 1 to 5 µl of antibody per 500 µg of cleared lysate.

Storage guidance: aliquot the 50 µl volume upon receipt and keep at -20°C in single-use fractions to limit repeated freeze-thaw cycles; protect the vial from light and keep it on ice during active bench use.