Anti-NSE/Enolase Antibody

Manufactured by Antibodies.com, this primary antibody is a rabbit polyclonal reagent raised against Neuron-Specific Enolase (NSE), also known as gamma-enolase or ENO2. It is supplied as an unconjugated liquid at a working concentration of 1 mg/mL in 50 µL aliquots, formulated in phosphate-buffered saline (pH 7.2) with 150 mM NaCl, 0.02% sodium azide as preservative, and 50% glycerol for cryoprotection. The antibody is affinity-purified from rabbit antiserum using an epitope-specific immunogen and has been validated for Western blot (WB) detection of the endogenous ~47 kDa NSE/ENO2 protein, with reactivity confirmed against human, mouse and rat samples. Recommended working dilutions begin at 1:500–1:1000 for WB applications, making it suitable for routine immunoblotting of cell and tissue lysates. The product should be stored at –20 °C for long-term stability, with aliquoting recommended to avoid repeated freeze-thaw cycles. Researchers working in neuroscience, cancer biomarker studies and glycolysis-related investigations can rely on this lot for sensitive and reproducible detection of the neuronal enolase isoform.