Anti-NSE Antibody

This polyclonal antibody from Antibodies.com targets Neuron-Specific Enolase (NSE), a glycolytic isoenzyme expressed predominantly in mature neurons and neuroendocrine cells. NSE, also known as gamma-enolase or ENO2, is widely used as an immunohistochemical marker for neuronal differentiation and serves as a clinically important serum biomarker for small cell lung carcinoma and other neuroendocrine tumors.

Raised in rabbit, this unconjugated reagent is supplied as 100 µl of affinity-purified antibody and has been validated for western blot, immunohistochemistry, and immunofluorescence workflows on human tissue samples. Researchers studying neurogenesis, neural crest development, neuroendocrine neoplasms, or brain tissue injury typically apply the antibody at working dilutions optimized for their specific detection chemistry.

The polyclonal format provides broader epitope coverage across the NSE molecule compared with monoclonal alternatives, which is particularly useful when working with formalin-fixed paraffin-embedded specimens where antigen masking may obscure individual epitopes. For research applications including immunoblotting of neuronal lysates and immunofluorescence staining of cultured neurons, the reagent supports clear visualization of the cytoplasmic NSE localization characteristic of mature neurons.

Storage recommendations include aliquoting upon receipt to preserve activity across multiple experiments; the antibody remains stable when stored frozen between uses and protected from light during handling. Intended for research use only, this reagent is not approved for clinical diagnostic procedures or therapeutic use.