Anti-Nrf2 (phospho-S40) Antibody

A recombinant rabbit monoclonal reagent from Antibodies.com supplied as a 50 µl vial, targeting phosphorylated Nrf2 at serine 40 in a TBS-based formulation (pH 7.4) with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative. This unconjugated primary antibody is validated for Western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, and flow cytometry applications on human samples.

Nrf2 (Nuclear factor erythroid 2-related factor 2, encoded by NFE2L2) is a master transcription factor governing the cellular antioxidant response through binding to antioxidant response elements in gene promoters. Phosphorylation at serine 40 modulates its nuclear accumulation and transcriptional activity, making phospho-S40 detection a useful marker for investigating KEAP1-NRF2 pathway activation under oxidative stress conditions.

Recombinant production supports batch-to-batch consistency across experiments. Researchers commonly use this antibody to study electrophile-induced signaling, chemoprotective gene expression, and crosstalk between Nrf2 and inflammatory pathways. Aliquot upon receipt to limit freeze-thaw cycles; the glycerol-containing buffer protects the protein during frozen storage.