Anti-NRF-1 Antibody

NRF-1 (Nuclear Respiratory Factor 1) is a transcription factor that drives mitochondrial biogenesis by activating nuclear-encoded genes required for oxidative phosphorylation, heme biosynthesis, and mtDNA replication. This rabbit polyclonal antibody, manufactured by Antibodies.com, detects endogenous NRF-1 in western blot and immunohistochemistry workflows using human, mouse, and rat samples.

Supplied unconjugated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the reagent is packaged as a 100 µl vial. Polyclonal reactivity broadens epitope coverage, which is useful when monitoring changes in NRF-1 abundance linked to metabolic stress, mitochondrial dysfunction, or aging-related pathways.

For western blot, whole-cell lysates prepared in RIPA buffer with protease inhibitors typically yield a band near 67 kDa, consistent with the full-length NRF-1 isoform. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, heat-induced antigen retrieval with citrate buffer (pH 6.0) is commonly used. Store at -20°C and divide into aliquots to limit freeze-thaw cycles, given the glycerol-containing formulation.